Nucleic Acid Extraction Or Purification Kit

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  • FOB Price: US $0.8 - 1 / Piece
  • Min.Order Quantity: 10000 Piece/Pieces
  • Supply Ability: 10000000 Piece/Pieces per Month
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    Product Detail

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    Nucleic Acid Extraction Or Purification Kit             or stored at -20℃. The sample should be are transported using 0℃ curling.

     

    Introduction

    The  Nucleic  Acid  Extraction  Or  Purification  Kit  (Magnetic  Beads Method) is designed for the automated purification of RNA and DNA from  body  fluids  (such  as  swabs,  plasma,  serum)  using  automated nucleic   acid   extraction   instruments.   Magnetic-particle  technology provides  high-quality  DNA/RNA  that  is  suitable  for  direct  use  in downstream  applications  such  as  amplification  or  other  enzymatic reactions.

    Application Range

    The  whole  blood,  plasma,  serum  and  other  tissue  samples  were directly lysed and digested. The released nucleic acid was selectively adsorbed by super paramagnetic nanometer magnetic beads. Then the protein, inorganic salt ions and organic impurities were removed by washing  solution.Finally, the nucleic acid was  eluted with eluent to obtain a pure nucleic acid solution.

    Kit Contents

    Cat. No.YXN-VIRAL01-32A-BRComponents
    -50A- 100A
    Size32Tes50Test100Test
    Lysis Buffer96 wellPre-pack 

    ed

    Plates

    2 piecces

    25ml50mlSurfactant and Tris
    Wash Buffer I★15ml★30mlHigh-salt solution
    Wash Buffer Il★6ml*2★12ml*2Low-salt solution
    Elution Buffer10ml20mlLow-salt solution
    MagaBio Reagent1.0ml2.0mlMagnetic     particles
    Handbook(=YXN-VIRAL01-32A-BR)111
    Notes:For YXN-VIRAL01-32A-BR-50A, add 15mL Absolute ethanol to ★15mL      Wash Buffer I before use; add 24mL Absolute ethanol to ★6mL Wash buffer Il before use.
    For YXN-VIRAL01-32A-BR-100A, add 30mL Absolute ethanol to ★30mL      Wash Buffer I before use; add 48mL Absolute ethanol to ★12mL Wash buffer Il before use.【Reagents to be prepared by the user】Please prepare the absolute ethanol (analytical grade) by yourself.

     

    Storage Conditions

    Upon arrival of the kit, kit components can be stored at room temperature (15 − 25°C). The reagents are stable for up to one year from the manufacturing date.

     

    Sample Requirements

    1. Applicable sample: Swabs, plasma, serum and whole blood etc.

    2. Sample storage and transportation: The sample should be tested immediately

     

    Materials and Devices Required but Not Provided

    1. Disposable powder-free gloves

    2. Biohazard container

    3. Pencil or per

     

    Procedure

    The following uses sample strip extraction swab lotion as an example to briefly explain the operation steps of the extraction reagent on the biological nucleic acid extraction instrument Bioer NPA-32P or SMART 32. For other sample  types, please  refer  to  the user  manual.  It  can  also be  operated  by customers according experimental acquirement:

    1. Reagent Preparation

    a. For YXN-VB03-32A-50A and YXN-VB03-32A-100A

    Add    500uL Lysis Buffer to the column 1 and 7 of the 2.2mL 96-deep-well plate, 500uL Wash Buffer I to the column 2 and 8, 500uL Wash Buffer II to the column 3, 4 and 9,10; 70uL Elution Buffer to the column 5 and 11, 180uL pure water and 20uL MagaBio Reagent to the column 6 and 12 (the magnetic beads should be mixed thoroughly before use),

    b. For YXN-VB03-32A

    Put the 96 well pre-packed reagents at room temperature. Shake 96-well plate upside  down  for  three  times,  and  tear  off the  plastic  bag.  Centrifuge  the pre-packed reagent for a few seconds (or swing by hand a few times) to avoid reagent adhering to the wall of the tubes. Tear off the aluminum foil film of 96-well plate and identify the direction of the plate (magnetic beads in column #6 and #12),

    2.Sample Extraction

    1. Add 300uL sample to the 96 well plate columns #1 and #7, please avoid cross-contamination,

    2. Place 96 deep well plate to the instrument, install the 8-strip tips on the instrument,

    3. Run the program according to the following procedures,

    4.  After  the  automatic  purification  is  over,  transfer  the  elution  Buffer  in columns 5 and 11 to a clean antinuclear 0.5mL centrifuge tube; if not using it immediately, please store at-20 °C.

    Performance Characteristics

    1. The extracted product is detected by high-sensitivity HBV DNA detection reagent to reach a sensitivity of 10 IU/mL. The extracted product is detected by high-sensitivity HCV RNA detection reagent to reach a sensitivity of 50 IU/mL.

    2. Select  4  samples  (serum/plasma  sample,  nasopharyngeal  swab  sample, cervical  exfoliated  cell  sample),  each  sample  is  diluted  10  times  with  3 gradients(including  the  original  sample  total  of  4  concentrations),  using qualified reagents and tests agents to detect internal reference gene according to the product instructions, and the Ct value of each batch differs by less than 1.

     

    StepWell LocationProgram NameWaiting       Time(min:SS)Mixing    Time(min:SS)Magnet       Time(min:SS)AdsorptionSpeedVolume tatus(μL)Temperature
    11Lysis0:002:000:00F70080
    26Beads0:000:150:15F200
    31Bind0:003:000:45F700
    42Wash10:000:300:30F500
    53Wash20:000:300:30F500
    64Wash30:000:300:30F500
    75Elution2:002:300:30F7080
    86Discard0:000:150:00F200

     

     

    Safety

    1. GENERAL SAFETY.

    Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document.

    1.1  Before  using  an  instrument  or  device,  read  and  understand  the  safety information provided in the user documentation provided by the manufacturer of the instrument or device.

    1.2 Before handling chemicals, read and understand all applicable Safety Data Sheets  (SDSs)  and  use  appropriate  personal  protective  equipment  (gloves, gowns,  eye protection,  etc.).  To  obtain  SDSs,  see  the  “Documentation  and Support” section in this document.

    2. Chemical safety

    GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below, and consult the relevant SDS for specific precautions  and  instructions:  Read  and  understand  the  Safety  Data  Sheets (SDSs) provided by the chemical manufacturer before you  store, handle, or work with any chemicals or hazardous materials..

    2.1  Minimize  contact  with  chemicals.  Wear  appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing).

    2.2 Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood).

    2.3 Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer’s cleanup procedures as recommended in the SDS.

    2.4 Handle chemical wastes in a fume hood.

    2.5 Ensure use of primary and secondary waste containers. (A primary waste

     

    container holds the immediate waste. A secondary container contains spills or leaks from the primary container. Both containers must be compatible with the waste material and meet federal,  state, and local requirements for container storage.).

    2.6 After emptying a waste container, seal it with the cap provided.

    2.7 Characterize (by analysis if necessary) the waste generated by the particular applications, reagents, and substrates used in your laboratory.

    2.8 Ensure that the waste is stored, transferred, transported, and disposed of according to all local, state/provincial, and/or national regulations.

    2.9 Radioactive or biohazardous materials may require special handling, and disposal limitations may apply.

    3.Biological hazard safety

    Potential Biohazard. Depending on the samples used on this instrument, the surface  may  be  considered  a  biohazard.  Use  appropriate  decontamination methods when working with biohazards.

    BIOHAZARD.  Biological  samples  such  as  tissues,  body  fluids,  infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations. Wear appropriate protective equipment, which includes but is not limited to: protective eyewear, face shield, clothing/lab coat, and gloves. All work should be conducted in properly equipped facilities using the appropriate safety  equipment  (for  example,  physical  containment  devices).  Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially infectious materials.

    Read and follow the applicable guidelines and/or regulatory requirements in the following:

    In  the  U.S.:  U.S.  Department  of Health  and  Human  Services  guidelines published in Biosafety in Microbiological and Biomedical Laboratories found at: www.cdc.gov/biosafety.

    Occupational   Safety   and   Health   Standards,   Bloodborne   Pathogens   (29 CFR§1910.1030), found at:

    www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html

    Your   company’s/institution’s   Biosafety   Program   protocols   for   working with/handling  potentially  infectious  materials.  Additional  information  about biohazard guidelines is available at: www.cdc.gov.

    In the EU: Check local guidelines and legislation on biohazard and biosafety precaution  and  refer  to  the  best  practices  published  in  the  World  Health Organization  (WHO)  Laboratory  Biosafety  Manual, third  edition,  found  at: www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.


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